Hotspot

trnT-ACC-psbD

LSC · 31331-32453 · 1123 bp

Back to Malcolmia

Pi 0.00178

MarkerSeek score 59.90

Primer available yes

Label trnT-ACC-psbD

Feature Metadata

label_name: trnT-ACC-psbD
feature_type: igs
region: LSC
length_bp: 1123
variable_sites: 2
pi: 0.00178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACC-psbD_p1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1276-1277 bp	1.0	79.3
trnT-ACC-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1279-1280 bp	1.0	79.2
trnT-ACC-psbD_p3	CCAAGACGGCCCTTTTAACT	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1298-1299 bp	1.0	79.1
trnT-ACC-psbD_p4	ACGGCCCTTTTAACTCAGTG	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1293-1294 bp	1.0	79.0
trnT-ACC-psbD_p5	GACGGCCCTTTTAACTCAGT	ACCTACAAAAACGAAGCGGT	50.0	45.0	57.8	58.0	1294-1295 bp	1.0	78.4