Hotspot

psbM-trnD-GAC

LSC · 28722-29711 · 990 bp

Back to Malcolmia

Pi 0.00303

MarkerSeek score 55.00

Primer available yes

Label psbM-trnD-GAC

Feature Metadata

label_name: psbM-trnD-GAC
feature_type: igs
region: LSC
length_bp: 990
variable_sites: 3
pi: 0.00303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GAC_p1	AGGAACGAGAATGAAGAGTGC	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	1107-1108 bp	1.0	68.2
psbM-trnD-GAC_p2	ACGAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	56.9	58.5	1103-1104 bp	1.0	67.5
psbM-trnD-GAC_p3	GGAACGAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.3	58.5	1106-1107 bp	1.0	66.0
psbM-trnD-GAC_p4	AACGAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.6	58.5	1104-1105 bp	1.0	65.3
psbM-trnD-GAC_p5	GGAACGAGAATGAAGAGTGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.5	58.5	1106-1107 bp	1.0	64.8