Hotspot

trnC-UGC-petN

LSC · 27351-27991 · 641 bp

Back to Malcolmia

Pi 0.00156

MarkerSeek score 59.60

Primer available yes

Label trnC-UGC-petN

Feature Metadata

label_name: trnC-UGC-petN
feature_type: igs
region: LSC
length_bp: 641
variable_sites: 1
pi: 0.00156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-UGC-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	751 bp	1.0	79.4
trnC-UGC-petN_p2	TTTCCCCAGTTCAAATCCGG	CTACCATTAAAGCCGCCCAT	50.0	50.0	58.1	58.0	710 bp	1.0	79.3
trnC-UGC-petN_p3	GTTTCTATCGTTTTGGCGGC	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	807 bp	1.0	79.2
trnC-UGC-petN_p4	GTTTCTATCGTTTTGGCGGC	CTACCATTAAAGCCGCCCAT	50.0	50.0	58.1	58.0	766 bp	1.0	79.1
trnC-UGC-petN_p5	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	752 bp	1.0	78.8