Hotspot

trnS-AGC-trnG-GGA

LSC · 7802-8452 · 651 bp

Back to Malcolmia

Pi 0.00307
MarkerSeek score 60.50
Primer available yes
Label trnS-AGC-trnG-GGA

Feature Metadata

label_name
trnS-AGC-trnG-GGA
feature_type
igs
region
LSC
length_bp
651
variable_sites
2
pi
0.00307
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-AGC-trnG-GGA_p1 ACGCTTTAATCCACTCAGCC TGAATCAAACCGAGAGACCC 50.0 50.0 58.3 57.2 758-762 bp 1.0 72.0
trnS-AGC-trnG-GGA_p2 CGCTTTAATCCACTCAGCCA TGAATCAAACCGAGAGACCC 50.0 50.0 58.3 57.2 757-761 bp 1.0 72.0
trnS-AGC-trnG-GGA_p3 ACGCTTTAATCCACTCAGCC GAATCAAACCGAGAGACCCT 50.0 50.0 58.3 56.9 757-761 bp 1.0 69.6
trnS-AGC-trnG-GGA_p4 CGCTTTAATCCACTCAGCCA GAATCAAACCGAGAGACCCT 50.0 50.0 58.3 56.9 756-760 bp 1.0 69.6
trnS-AGC-trnG-GGA_p5 ATCCACTCAGCCATCTCTCC TGAATCAAACCGAGAGACCC 55.0 50.0 58.6 57.2 750-754 bp 1.0 69.5