Hotspot

psaC-ndhE

SSC · 119687-120060 · 374 bp

Back to Maianthemum

Pi 0.0199

MarkerSeek score 72.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 374
variable_sites: 30
pi: 0.0199
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	GGAAGCAGCTATTGGGCTAG	50.0	55.0	58.1	58.1	567-592 bp	1.0	83.4
psaC-ndhE_p2	CCAATACATCTGTGGGGCAA	GGAAGCAGCTATTGGGCTAG	50.0	55.0	57.9	58.1	544-569 bp	1.0	83.4
psaC-ndhE_p3	AATACATCTGTGGGGCAAGC	GGAAGCAGCTATTGGGCTAG	50.0	55.0	58.2	58.1	542-567 bp	1.0	82.7
psaC-ndhE_p4	AGCTTTACATCCGTCCCAAG	GGAAGCAGCTATTGGGCTAG	50.0	55.0	57.6	58.1	573-598 bp	1.0	81.1
psaC-ndhE_p5	TTCCAATACATCTGTGGGGC	GGAAGCAGCTATTGGGCTAG	50.0	55.0	57.6	58.1	546-571 bp	1.0	81.1