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psaJ-rpl33

LSC · 68728-69309 · 582 bp

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Pi 0.0233

MarkerSeek score 72.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 582
variable_sites: 38
pi: 0.0233
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTGGGTCTTTAGCGGGTCTA	GCCTGGCGATTCCTTATTGA	50.0	50.0	58.1	58.0	705-773 bp	1.0	87.4
psaJ-rpl33_p2	TTGGGTCTTTAGCGGGTCTA	TAGAAATGCCTGGCGATTCC	50.0	50.0	58.1	58.0	712-780 bp	1.0	87.4
psaJ-rpl33_p3	ATGGTTTGGGTCTTTAGCGG	GCCTGGCGATTCCTTATTGA	50.0	50.0	58.2	58.0	710-778 bp	1.0	86.5
psaJ-rpl33_p4	ATGGTTTGGGTCTTTAGCGG	TAGAAATGCCTGGCGATTCC	50.0	50.0	58.2	58.0	717-785 bp	1.0	86.5
psaJ-rpl33_p5	TGGGTCTTTAGCGGGTCTAT	GCCTGGCGATTCCTTATTGA	50.0	50.0	57.8	58.0	704-772 bp	1.0	86.5