Hotspot

ndhC-trnV-UAC

LSC · 51077-52740 · 1664 bp

Back to Maianthemum

Pi 0.0106

MarkerSeek score 54.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1664
variable_sites: 48
pi: 0.0106
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTGAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	36.4	55.0	56.1	58.7	1700-1730 bp	1.0	46.4
ndhC-trnV-UAC_p2	TCATATTCGTGAAGCAGAAACA	TTCGAGTCCGTATAGCCCTA	36.4	50.0	56.1	57.1	1699-1729 bp	1.0	44.3
ndhC-trnV-UAC_p3	CATATTCGTGAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	38.1	55.0	54.6	58.7	1699-1729 bp	1.0	42.9
ndhC-trnV-UAC_p4	ATCATATTCGTGAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	34.8	55.0	56.6	58.7	1701-1731 bp	1.0	42.9
ndhC-trnV-UAC_p5	TCATATTCGTGAAGCAGAAACAT	GTTCGAGTCCGTATAGCCCT	34.8	55.0	56.6	58.7	1700-1730 bp	1.0	42.9