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trnC-GCA-petN

LSC · 28569-29492 · 924 bp

Back to Maianthemum

Pi 0.0143

MarkerSeek score 60.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 924
variable_sites: 36
pi: 0.0143
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTA	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.5	58.0	1000-1014 bp	1.0	79.4
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTA	AGTCCACTCCTTCCCCATAC	50.0	55.0	57.5	58.1	1052-1066 bp	1.0	78.3
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTA	GTCCACTCCTTCCCCATACT	50.0	55.0	57.5	58.1	1051-1065 bp	1.0	78.3
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTA	GAGTCCACTCCTTCCCCATA	50.0	55.0	57.5	57.8	1053-1067 bp	1.0	77.9
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTA	AGCAATTAGTAGTGCCCAGC	50.0	50.0	57.5	58.0	80-1082 bp	0.888889	74.4