Hotspot

rpoB-trnC-GCA

LSC · 27238-28497 · 1260 bp

Back to Maianthemum

Pi 0.0121

MarkerSeek score 58.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1260
variable_sites: 47
pi: 0.0121
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGAGGTTCCCAGGGAATTCA	AGAATCAGGAGAACCCACCA	50.0	50.0	57.9	58.0	2189-2209 bp	1.0	83.4
rpoB-trnC-GCA_p2	GAAAATACCGGATCCCCACC	AGAATCAGGAGAACCCACCA	55.0	50.0	58.0	58.0	2569-2589 bp	1.0	83.1
rpoB-trnC-GCA_p3	GAAAATACCGGATCCCCACC	AATCGAGTATTGACACGCCC	55.0	50.0	58.0	58.1	2153-2171 bp	1.0	82.9
rpoB-trnC-GCA_p4	GAAAATACCGGATCCCCACC	ATTGACACGCCCACTTAGTC	55.0	50.0	58.0	57.9	2145-2163 bp	1.0	82.6
rpoB-trnC-GCA_p5	GAAAATACCGGATCCCCACC	GAGTATTGACACGCCCACTT	55.0	50.0	58.0	57.9	2149-2159 bp	0.888889	77.3