Hotspot

atpF-atpH

LSC · 13726-14218 · 493 bp

Back to Maianthemum

Pi 0.0117

MarkerSeek score 67.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 493
variable_sites: 18
pi: 0.0117
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.9	58.0	80-625 bp	1.0	85.5
atpF-atpH_p2	CAGTGGCCTAAGGAAACGAA	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.8	58.0	311-597 bp	1.0	84.8
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.9	58.3	326-612 bp	1.0	83.4
atpF-atpH_p4	CAGTGGCCTAAGGAAACGAA	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.8	58.3	298-584 bp	1.0	83.0
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CGTGGCATTAGCGCTTTTAT	50.0	45.0	57.9	57.2	325-611 bp	1.0	79.5