Hotspot

trnS-GCU-trnG-UCC

LSC · 8375-9662 · 1288 bp

Back to Maianthemum

Pi 0.0179

MarkerSeek score 65.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1288
variable_sites: 63
pi: 0.0179
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATCAGAGGGAACGGAGAGAG	TACCACTAAACTATACCCGC	55.0	45.0	57.9	54.0	1282-1464 bp	1.0	55.0
trnS-GCU-trnG-UCC_p2	ATCAGAGGGAACGGAGAGAG	TTACCACTAAACTATACCCGC	55.0	42.9	57.9	54.9	1283-1465 bp	1.0	53.9
trnS-GCU-trnG-UCC_p3	GGAACGGAGAGAGAGGGATT	TACCACTAAACTATACCCGC	55.0	45.0	58.2	54.0	1275-1457 bp	1.0	53.8
trnS-GCU-trnG-UCC_p4	AATCTGCCGCTTTAGTCCAC	TACCACTAAACTATACCCGC	50.0	45.0	58.3	54.0	1217-1399 bp	1.0	53.6
trnS-GCU-trnG-UCC_p5	CAATCTGCCGCTTTAGTCCA	TACCACTAAACTATACCCGC	50.0	45.0	58.3	54.0	1218-1400 bp	1.0	53.6