Hotspot

psbI-trnS-GCU

LSC · 8164-8286 · 123 bp

Back to Maianthemum

Pi 0.0379
MarkerSeek score 72.80
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
123
variable_sites
12
pi
0.0379
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CTTATCTAACGATCCGGGCC ATTGGGAGAGATGGCTGAGT 55.0 50.0 57.6 58.1 254-2225 bp 1.0 78.9
psbI-trnS-GCU_p2 CTTATCTAACGATCCGGGCC GGGTTCGAATCCCTCTCTCT 55.0 55.0 57.6 58.2 185-2156 bp 1.0 78.5
psbI-trnS-GCU_p3 CTTATCTAACGATCCGGGCC GTGGACTAAAGCGGCAGATT 55.0 50.0 57.6 58.3 236-2207 bp 1.0 77.8
psbI-trnS-GCU_p4 CTTATCTAACGATCCGGGCC TGGACTAAAGCGGCAGATTG 55.0 50.0 57.6 58.3 235-2206 bp 1.0 77.8
psbI-trnS-GCU_p5 CTTATCTAACGATCCGGGCC TTCGAATCCCTCTCTCTCCG 55.0 55.0 57.6 58.3 182-2153 bp 1.0 77.7