Hotspot

psbB

IRa · 164129-165655 · 1527 bp

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Pi 0

MarkerSeek score 26.40

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: IRa
length_bp: 1527
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	CCACACCTGTTCATTTGGGA	TTATTTGATAAAGGGGTATTTTC	50.0	26.1	58.0	50.5	1706 bp	0.833333	33.4
psbB_p2	TCCACACCTGTTCATTTGGG	TTATTTGATAAAGGGGTATTTTC	50.0	26.1	58.0	50.5	1707 bp	0.833333	33.4
psbB_p3	CCTTAGGCGGTTCTCGAAAA	TTATTTGATAAAGGGGTATTTTC	50.0	26.1	57.9	50.5	1821 bp	0.833333	33.4
psbB_p4	CCACACCTGTTCATTTGGGA	TTTATTTGATAAAGGGGTATTTTC	50.0	25.0	58.0	51.5	1707 bp	0.833333	33.4
psbB_p5	TCCACACCTGTTCATTTGGG	TTTATTTGATAAAGGGGTATTTTC	50.0	25.0	58.0	51.5	1708 bp	0.833333	33.4