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ccsA-ndhD

SSC · 115741-115976 · 236 bp

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Pi 0.0412

MarkerSeek score 65.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 236
variable_sites: 19
pi: 0.0412
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGCAATTGTAGCTTCTATGGGA	TGCACTATCAGTTGACAAGGT	40.9	42.9	57.5	57.2	403-404 bp	1.0	49.6
ccsA-ndhD_p2	TGCAATTGTAGCTTCTATGGGA	TCCGGATTTTGTTTTTGCACT	40.9	38.1	57.5	57.2	418-419 bp	1.0	49.1
ccsA-ndhD_p3	TGCAATTGTAGCTTCTATGGGA	CGATCTGTATCCTTCTACCCG	40.9	52.4	57.5	57.2	458-459 bp	1.0	48.6
ccsA-ndhD_p4	TGCAATTGTAGCTTCTATGGGA	TTGCACTATCAGTTGACAAGGT	40.9	40.9	57.5	57.9	404-405 bp	1.0	47.1
ccsA-ndhD_p5	CTGCAATTGTAGCTTCTATGGG	TGCACTATCAGTTGACAAGGT	45.5	42.9	57.2	57.2	404-405 bp	1.0	47.0