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rps12-clpP

LSC · 70629-71117 · 489 bp

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Pi 0.00927

MarkerSeek score 52.20

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 489
variable_sites: 13
pi: 0.00927
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	GTACATGTTCCTCGACGCTG	ACTTTTATGGACGCAAGCGA	55.0	45.0	58.7	58.2	691-698 bp	1.0	75.1
rps12-clpP_p2	AGTACATGTTCCTCGACGCT	ACTTTTATGGACGCAAGCGA	50.0	45.0	58.8	58.2	692-699 bp	1.0	74.4
rps12-clpP_p3	TACATGTTCCTCGACGCTGA	ACTTTTATGGACGCAAGCGA	50.0	45.0	58.8	58.2	690-697 bp	1.0	74.3
rps12-clpP_p4	TAGTACATGTTCCTCGACGC	ACTTTTATGGACGCAAGCGA	50.0	45.0	57.2	58.2	693-700 bp	1.0	74.2
rps12-clpP_p5	AAGAGCGGGGGATTTTGTG	ACTTTTATGGACGCAAGCGA	52.6	45.0	58.0	58.2	659-666 bp	1.0	72.8