Hotspot

rpoB-trnC-GCA

LSC · 26463-27664 · 1202 bp

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Pi 0.00294

MarkerSeek score 61.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1202
variable_sites: 10
pi: 0.00294
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGTCAAGCCCTGATCAATGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1357-1367 bp	1.0	79.9
rpoB-trnC-GCA_p2	CGTCAAGCCCTGATCAATGA	TGGGGAAAAAGGGATTTGCA	50.0	45.0	58.0	57.9	1345-1355 bp	1.0	79.7
rpoB-trnC-GCA_p3	CGTCAAGCCCTGATCAATGA	TTATTGATCAGGCGACACCC	50.0	50.0	58.0	57.7	1375-1385 bp	1.0	78.0
rpoB-trnC-GCA_p4	AAAGTTCTTCCGTCAAGCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1367-1377 bp	1.0	77.0
rpoB-trnC-GCA_p5	AAAGTTCTTCCGTCAAGCCC	TGGGGAAAAAGGGATTTGCA	50.0	45.0	58.4	57.9	1355-1365 bp	1.0	76.8