Hotspot

trnS-GCU-trnR-UCU

LSC · 8033-9743 · 1711 bp

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Pi 0.000723

MarkerSeek score 56.00

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1711
variable_sites: 3
pi: 0.000723
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	ATTAGCAATCCGCCGCTTTA	GATGGGAAAATTTGCGTCCA	45.0	45.0	58.0	57.0	1807-1835 bp	1.0	73.0
trnS-GCU-trnR-UCU_p2	ACGGAAAGAGAGGGATTCGA	GATGGGAAAATTTGCGTCCA	50.0	45.0	58.2	57.0	1856-1884 bp	1.0	72.0
trnS-GCU-trnR-UCU_p3	ATTAGCAATCCGCCGCTTTA	GATGGGAAAATTTGCGTCCAA	45.0	42.9	58.0	57.7	1807-1835 bp	1.0	70.7
trnS-GCU-trnR-UCU_p4	ATTAGCAATCCGCCGCTTTA	AGATGGGAAAATTTGCGTCC	45.0	45.0	58.0	56.7	1808-1836 bp	1.0	70.6
trnS-GCU-trnR-UCU_p5	GCTTTAGTCCACTCAGCCAT	GATGGGAAAATTTGCGTCCA	50.0	45.0	57.6	57.0	1793-1821 bp	1.0	70.0