Hotspot

trnK-UUU-rps16

LSC · 4419-5009 · 591 bp

Back to Mahonia

Pi 0.00575
MarkerSeek score 65.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
591
variable_sites
9
pi
0.00575
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCTACCAACGGTATGGACGA AAAAAGGCGCTCAACCTACA 50.0 45.0 57.9 58.0 746-758 bp 1.0 81.3
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 681-693 bp 1.0 81.3
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 680-692 bp 1.0 81.3
trnK-UUU-rps16_p4 TCTAGCCGCACTTAAAAGCC AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 695-707 bp 1.0 80.4
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 690-702 bp 1.0 80.3