Hotspot

ycf1

SSC · 128912-134506 · 5595 bp

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Pi 0.00738

MarkerSeek score 58.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5595
variable_sites: 529
pi: 0.00738
unique_haplotype_count: 131
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCACCTCTTGTGCTTGATCA	AGGCACAACTTGAGCAATGA	50.0	45.0	57.8	58.0	80-862 bp	0.974026	89.3
ycf1_p2	TATCAACGCCACCTCTTGTG	AGGCACAACTTGAGCAATGA	50.0	45.0	57.9	58.0	80-852 bp	0.467532	69.0
ycf1_p3	GTATCAACGCCACCTCTTGT	AGGCACAACTTGAGCAATGA	50.0	45.0	57.9	58.0	80-853 bp	0.467532	68.9
ycf1_p4	TATCAACGCCACCTCTTGTG	GCTGGGGAATCAACACCTAG	50.0	55.0	57.9	58.0	80-486 bp	0.474026	68.7
ycf1_p5	GTATCAACGCCACCTCTTGT	GCTGGGGAATCAACACCTAG	50.0	55.0	57.9	58.0	80-487 bp	0.474026	68.7