Hotspot

rpl32-trnL-UAG

SSC · 118033-119237 · 1205 bp

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Pi 0.0131
MarkerSeek score 62.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1205
variable_sites
206
pi
0.0131
unique_haplotype_count
127
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ATCAACAACAACCGGTTCGA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 80-1811 bp 0.967532 90.3
rpl32-trnL-UAG_p2 TCAACAACAACCGGTTCGAT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 80-1810 bp 0.967532 90.3
rpl32-trnL-UAG_p3 ATCAACAACAACCGGTTCGA CAGCGTGTCTACCGATTTCA 45.0 50.0 58.0 58.0 80-1812 bp 0.967532 90.3
rpl32-trnL-UAG_p4 TCAACAACAACCGGTTCGAT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.0 58.0 80-1811 bp 0.967532 90.3
rpl32-trnL-UAG_p5 TCAACAACAACCGGTTCGAT TCCTTTTTAGAGGATGCCGC 45.0 50.0 58.0 58.0 80-1875 bp 0.967532 90.0