Hotspot

ndhF-rpl32

SSC · 116717-117858 · 1142 bp

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Pi 0.0121
MarkerSeek score 71.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1142
variable_sites
167
pi
0.0121
unique_haplotype_count
104
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CAGTCGGAACAAGCAGAAGT GATAAAGCTTTTACCGCCGC 50.0 50.0 58.1 57.9 80-1362 bp 0.993506 89.9
ndhF-rpl32_p2 AGTCGGAACAAGCAGAAGTC GATAAAGCTTTTACCGCCGC 50.0 50.0 57.8 57.9 80-1361 bp 0.993506 89.7
ndhF-rpl32_p3 CTGGAAGTGGAACGAAAGGT GATAAAGCTTTTACCGCCGC 50.0 50.0 57.7 57.9 80-1320 bp 0.993506 88.9
ndhF-rpl32_p4 AACAAGCAGAAGTCCCCATC GATAAAGCTTTTACCGCCGC 50.0 50.0 57.8 57.9 80-1355 bp 0.935065 86.9
ndhF-rpl32_p5 GAACAAGCAGAAGTCCCCAT GATAAAGCTTTTACCGCCGC 50.0 50.0 57.8 57.9 80-1356 bp 0.935065 86.9