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psaJ-rpl33

LSC · 70904-71334 · 431 bp

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Pi 0.0106

MarkerSeek score 61.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 431
variable_sites: 51
pi: 0.0106
unique_haplotype_count: 121
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GATGCGTTGGTATTCCCCTT	GACATCTTTACCCTTGGCCA	50.0	50.0	57.9	57.5	80-507 bp	0.993506	87.3
psaJ-rpl33_p2	GATGCGTTGGTATTCCCCTT	TGACATCTTTACCCTTGGCC	50.0	50.0	57.9	57.5	80-508 bp	0.993506	87.3
psaJ-rpl33_p3	TGGTTTGGGTCTTTAGCAGG	GACATCTTTACCCTTGGCCA	50.0	50.0	57.7	57.5	80-558 bp	0.974026	84.5
psaJ-rpl33_p4	TGGTTTGGGTCTTTAGCAGG	TGACATCTTTACCCTTGGCC	50.0	50.0	57.7	57.5	80-559 bp	0.974026	84.5
psaJ-rpl33_p5	GATGCGTTGGTATTCCCCTT	TCATTTCGGACACAACTGGT	50.0	45.0	57.9	57.4	80-551 bp	0.922078	83.4