Hotspot

ycf3-trnS-GGA

LSC · 46768-47201 · 434 bp

Back to Magnolia

Pi 0.00672

MarkerSeek score 61.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 434
variable_sites: 43
pi: 0.00672
unique_haplotype_count: 52
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.1	57.8	534-660 bp	1.0	82.1
ycf3-trnS-GGA_p2	GCCTCCTTTTCTCCTGAAGT	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.1	57.8	533-659 bp	1.0	82.1
ycf3-trnS-GGA_p3	TGCCTCCTTTTCTCCTGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.1	58.2	584-710 bp	1.0	81.9
ycf3-trnS-GGA_p4	GCCTCCTTTTCTCCTGAAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.1	58.2	583-709 bp	1.0	81.9
ycf3-trnS-GGA_p5	GCCTCCTTTTCTCCTGAAGTT	ACGGAAAGAGAGGGATTCGA	47.6	50.0	57.9	58.2	583-709 bp	1.0	79.6