Hotspot

petN-psbM

LSC · 30196-30942 · 747 bp

Back to Magnolia

Pi 0.00923

MarkerSeek score 68.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 100
pi: 0.00923
unique_haplotype_count: 74
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	890-1223 bp	0.993506	88.5
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	891-1224 bp	0.993506	87.5
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.6	58.3	829-1162 bp	1.0	87.1
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TTGCTACTGCGCTGTTCATT	50.0	45.0	58.2	58.5	889-1222 bp	0.993506	86.7
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	57.6	57.6	830-1163 bp	1.0	86.2