Hotspot

ycf1

SSC · 112010-117601 · 5592 bp

Pi 0.00668

MarkerSeek score 53.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5592
variable_sites: 56
pi: 0.00668
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTACTGATACCGATCGCCG	TGCGCATTTGCTTGAAAGAG	55.0	45.0	58.0	57.9	587 bp	1.0	80.8
ycf1_p2	CTTACTGATACCGATCGCCG	GCGCATTTGCTTGAAAGAGT	55.0	45.0	58.0	57.9	586 bp	1.0	80.8
ycf1_p3	ACGACGAGTTGAAAAACCGA	TGCGCATTTGCTTGAAAGAG	45.0	45.0	58.1	57.9	826 bp	1.0	80.5
ycf1_p4	TTCTGGATGGGGATGGGAAT	TGCGCATTTGCTTGAAAGAG	50.0	45.0	58.1	57.9	1186 bp	1.0	80.4
ycf1_p5	TTCTGGATGGGGATGGGAAT	GCGCATTTGCTTGAAAGAGT	50.0	45.0	58.1	57.9	1185 bp	1.0	80.4