Hotspot

trnL-UAG-rpl32

SSC · 127274-127705 · 432 bp

Back to Maesa

Pi 0.0216
MarkerSeek score 61.70
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
432
variable_sites
12
pi
0.0216
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 TCCTAAGAGCAGCGTGTCTA TCTCTTTCCACCGGATATTCA 50.0 42.9 57.9 56.2 519-525 bp 1.0 59.5
trnL-UAG-rpl32_p2 TCCTAAGAGCAGCGTGTCTA GAAATCTCTTTCCACCGGAT 50.0 45.0 57.9 55.1 523-529 bp 1.0 59.2
trnL-UAG-rpl32_p3 AGCAGCGTGTCTACCAATTT TCTCTTTCCACCGGATATTCA 45.0 42.9 57.8 56.2 512-518 bp 1.0 59.0
trnL-UAG-rpl32_p4 CCTAAGAGCAGCGTGTCTAC TCTCTTTCCACCGGATATTCA 55.0 42.9 57.8 56.2 518-524 bp 1.0 58.9
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC TCTCTTTCCACCGGATATTCA 55.0 42.9 57.8 56.2 517-523 bp 1.0 58.9