Hotspot

ndhD-ccsA

SSC · 125855-126095 · 241 bp

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Pi 0.0166
MarkerSeek score 63.10
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
241
variable_sites
6
pi
0.0166
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 AGTTGACAAGGTCGAAGTTGT CCATTGTGGCTTCTATTGGC 42.9 50.0 57.7 57.1 406 bp 1.0 64.1
ndhD-ccsA_p2 TCAGTTGACAAGGTCGAAGT CCATTGTGGCTTCTATTGGC 45.0 50.0 56.7 57.1 408 bp 1.0 64.0
ndhD-ccsA_p3 TCAGTTGACAAGGTCGAAGTT CCATTGTGGCTTCTATTGGC 42.9 50.0 57.5 57.1 408 bp 1.0 61.9
ndhD-ccsA_p4 CAGTTGACAAGGTCGAAGTTG CCATTGTGGCTTCTATTGGC 47.6 50.0 57.4 57.1 407 bp 1.0 61.4
ndhD-ccsA_p5 ATCAGTTGACAAGGTCGAAGT CCATTGTGGCTTCTATTGGC 42.9 50.0 57.2 57.1 409 bp 1.0 60.1