Hotspot

psbE-petL

LSC · 67692-68806 · 1115 bp

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Pi 0.00998
MarkerSeek score 64.00
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1115
variable_sites
16
pi
0.00998
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GTCGTATCTTGCTCAGACCA 50.0 50.0 57.9 57.1 1294-1307 bp 1.0 73.8
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC GTCGTATCTTGCTCAGACCA 50.0 50.0 57.8 57.1 1217-1230 bp 1.0 73.1
psbE-petL_p3 ACGAATAACCAACCCGCAAT GTCGTATCTTGCTCAGACCA 45.0 50.0 58.2 57.1 1303-1316 bp 1.0 72.9
psbE-petL_p4 TGCTGACGAATAACCAACCC GTCGTATCTTGCTCAGACCA 50.0 50.0 58.2 57.1 1308-1321 bp 1.0 72.8
psbE-petL_p5 CGAATAACCAACCCGCAATG GTCGTATCTTGCTCAGACCA 50.0 50.0 57.8 57.1 1302-1315 bp 1.0 72.7