Hotspot

rbcL-accD

LSC · 59131-59741 · 611 bp

Back to Maesa

Pi 0.00438
MarkerSeek score 61.80
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
611
variable_sites
4
pi
0.00438
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGGCTAGCAAATGGAGTCC ACCCACACCTGTGTTCTAAC 55.0 50.0 58.0 57.4 761-762 bp 1.0 75.2
rbcL-accD_p2 GCTGCTTGTGAGGTATGGAA ACCCACACCTGTGTTCTAAC 50.0 50.0 57.9 57.4 731-732 bp 1.0 74.7
rbcL-accD_p3 TGCTCGTGAGGGTAATGAGA ACCCACACCTGTGTTCTAAC 50.0 50.0 58.2 57.4 789-790 bp 1.0 74.3
rbcL-accD_p4 GAGTCCTGAACTAGCTGCTG ACCCACACCTGTGTTCTAAC 55.0 50.0 57.7 57.4 747-748 bp 1.0 73.2
rbcL-accD_p5 GGCTAGCAAATGGAGTCCTG ACCCACACCTGTGTTCTAAC 55.0 50.0 58.3 57.4 759-760 bp 1.0 73.0