Hotspot

trnL-UAA-trnF-GAA

LSC · 49902-50280 · 379 bp

Back to Maesa

Pi 0.0158
MarkerSeek score 54.70
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
379
variable_sites
9
pi
0.0158
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AATCGTGAGGGTTCAAGTCC TCTACCAGCTGAGCTATCCC 50.0 55.0 57.5 58.0 428-433 bp 1.0 78.8
trnL-UAA-trnF-GAA_p2 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 464-469 bp 1.0 78.2
trnL-UAA-trnF-GAA_p3 AATCGTGAGGGTTCAAGTCC GTGCCAGGAACCAGATTTGA 50.0 50.0 57.5 58.1 479-484 bp 1.0 77.8
trnL-UAA-trnF-GAA_p4 AATCGTGAGGGTTCAAGTCC TCAGTCCTCTGCTCTACCAG 50.0 55.0 57.5 57.9 440-445 bp 1.0 77.6
trnL-UAA-trnF-GAA_p5 AATCGTGAGGGTTCAAGTCC CATGTGCCAGGAACCAGATT 50.0 50.0 57.5 58.2 482-487 bp 1.0 77.3