Hotspot

atpA-atpF

LSC · 12271-12324 · 54 bp

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Pi 0.0123
MarkerSeek score 64.60
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
54
variable_sites
1
pi
0.0123
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 TTCGTCGGCTCGAATAGTTG AGGTGCTCTAGGAACTCTGA 50.0 50.0 58.1 57.1 182-190 bp 1.0 73.3
atpA-atpF_p2 TTCGTCGGCTCGAATAGTTG TTACGTACCATCAGTGCCAA 50.0 45.0 58.1 56.9 136-144 bp 1.0 71.9
atpA-atpF_p3 TTCGTCGGCTCGAATAGTTG TTTACGTACCATCAGTGCCA 50.0 45.0 58.1 56.9 137-145 bp 1.0 71.9
atpA-atpF_p4 TTCGTCGGCTCGAATAGTTG AAGGTGCTCTAGGAACTCTGA 50.0 47.6 58.1 57.8 183-191 bp 1.0 71.1
atpA-atpF_p5 TTCGTCGGCTCGAATAGTTG AGGTGCTCTAGGAACTCTGAA 50.0 47.6 58.1 57.8 182-190 bp 1.0 71.1