Hotspot

psbK-psbI

LSC · 8237-8651 · 415 bp

Back to Maesa

Pi 0.00811
MarkerSeek score 61.20
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
415
variable_sites
5
pi
0.00811
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 CAAGCTGCCGTAAGTTTTCG ACGCCCTGGATCGTTAGATA 50.0 50.0 58.0 58.0 524-529 bp 1.0 81.1
psbK-psbI_p2 CAAGCTGCCGTAAGTTTTCG TACGCCCTGGATCGTTAGAT 50.0 50.0 58.0 58.0 525-530 bp 1.0 81.1
psbK-psbI_p3 CAAGCTGCCGTAAGTTTTCG TTACGCCCTGGATCGTTAGA 50.0 50.0 58.0 58.2 526-531 bp 1.0 79.3
psbK-psbI_p4 GCAAGCTGCCGTAAGTTTTC ACGCCCTGGATCGTTAGATA 50.0 50.0 58.3 58.0 525-530 bp 1.0 78.9
psbK-psbI_p5 GCAAGCTGCCGTAAGTTTTC TACGCCCTGGATCGTTAGAT 50.0 50.0 58.3 58.0 526-531 bp 1.0 78.9