Hotspot

rps8

LSC · 80510-80914 · 405 bp

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Pi 0.0198
MarkerSeek score 54.00
Primer available yes
Label rps8

Feature Metadata

label_name
rps8
feature_type
gene
region
LSC
length_bp
405
variable_sites
8
pi
0.0198
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8_p1 AGTTCGAGATACAATTTTGATTTTGA AAAATTCGGAGCAAGGGGAA 26.9 45.0 56.2 57.7 474 bp 0.5 20.0
rps8_p2 AGTTCGAGATACAATTTTGATTTTGA TTCGGAGCAAGGGGAATTTT 26.9 45.0 56.2 57.7 470 bp 0.5 20.0
rps8_p3 AGTTCGAGATACAATTTTGATTTTGA CAAAATTCGGAGCAAGGGGA 26.9 50.0 56.2 58.5 475 bp 0.5 20.0
rps8_p4 AGTTCGAGATACAATTTTGATTTTGA TCAAAATTCGGAGCAAGGGG 26.9 50.0 56.2 58.5 476 bp 0.5 20.0
rps8_p5 AGTTCGAGATACAATTTTGATTTTGA ATTCGGAGCAAGGGGAATTT 26.9 45.0 56.2 57.5 471 bp 0.5 20.0