Hotspot

ndhA

SSC · 120911-123053 · 2143 bp

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Pi 0.0235
MarkerSeek score 64.90
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2143
variable_sites
50
pi
0.0235
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 CCCGGTTATCATAGGAAGCA TGACGATACTCGGTAGCATAGA 50.0 45.5 56.8 58.0 2297-2310 bp 1.0 56.4
ndhA_p2 ACCCGGTTATCATAGGAAGC TGACGATACTCGGTAGCATAGA 50.0 45.5 56.8 58.0 2298-2311 bp 1.0 56.4
ndhA_p3 CCCGGTTATCATAGGAAGCA ATGACGATACTCGGTAGCAT 50.0 45.0 56.8 55.9 2298-2311 bp 1.0 56.0
ndhA_p4 ACCCGGTTATCATAGGAAGC ATGACGATACTCGGTAGCAT 50.0 45.0 56.8 55.9 2299-2312 bp 1.0 55.9
ndhA_p5 AACCCGGTTATCATAGGAAGC TGACGATACTCGGTAGCATAGA 47.6 45.5 57.5 58.0 2299-2312 bp 1.0 54.3