Hotspot

ccsA

SSC · 115536-116516 · 981 bp

Pi 0.0173

MarkerSeek score 55.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 981
variable_sites: 17
pi: 0.0173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTTTTGAACAAGTTGCCGCT	CTTTGATTCTGGACCCCGAG	45.0	55.0	58.1	58.0	1629-1668 bp	1.0	84.2
ccsA_p2	AAGTTGCCGCTATGGTGAAA	CTTTGATTCTGGACCCCGAG	45.0	55.0	58.1	58.0	1620-1659 bp	1.0	84.1
ccsA_p3	GTTTTGAACAAGTTGCCGCT	TTATCGATGGCTTCCCTTGC	45.0	50.0	58.1	58.0	1850-1889 bp	1.0	83.7
ccsA_p4	AAGTTGCCGCTATGGTGAAA	TTATCGATGGCTTCCCTTGC	45.0	50.0	58.1	58.0	1841-1880 bp	1.0	83.6
ccsA_p5	GTTTTGAACAAGTTGCCGCT	CGAAATGGGTGGAATGGCTA	45.0	50.0	58.1	57.9	1905-1944 bp	1.0	83.5