Hotspot

rps15-ycf1

SSC · 124606-124998 · 393 bp

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Pi 0.0256
MarkerSeek score 56.10
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
393
variable_sites
10
pi
0.0256
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 GCTGGCCCTTTTAGGAACTT TCCTTTGGCCCAATTTTCGA 50.0 45.0 58.1 58.0 1182-1184 bp 1.0 81.4
rps15-ycf1_p2 GCTGGCCCTTTTAGGAACTT TCAAGTTCTTCCTTTGGCCC 50.0 50.0 58.1 58.0 1191-1193 bp 1.0 81.4
rps15-ycf1_p3 GCTGGCCCTTTTAGGAACTT GCGTTTTCAATTGCGATGGA 50.0 45.0 58.1 58.0 1778-1786 bp 1.0 81.4
rps15-ycf1_p4 GGTCCGAGCCATAACAGATG TCCTTTGGCCCAATTTTCGA 55.0 45.0 58.1 58.0 1244-1246 bp 1.0 80.9
rps15-ycf1_p5 GGTCCGAGCCATAACAGATG TCAAGTTCTTCCTTTGGCCC 55.0 50.0 58.1 58.0 1253-1255 bp 1.0 80.9