Hotspot

ccsA-ndhD

SSC · 116517-116846 · 330 bp

Back to Maerua

Pi 0.0925
MarkerSeek score 77.50
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
330
variable_sites
26
pi
0.0925
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGCTATTTTGGGGTCAATCTCT ACTAGCAAGTGACAAGGTCG 40.9 50.0 57.4 57.6 416-448 bp 1.0 64.6
ccsA-ndhD_p2 TGCTATTTTGGGGTCAATCTCT AGCAAGTGACAAGGTCGAAT 40.9 45.0 57.4 57.4 413-445 bp 1.0 63.7
ccsA-ndhD_p3 TGCTATTTTGGGGTCAATCTCT CTAGCAAGTGACAAGGTCGA 40.9 50.0 57.4 57.3 415-447 bp 1.0 62.3
ccsA-ndhD_p4 TGGGGTCAATCTCTTAGGAA ACTAGCAAGTGACAAGGTCG 45.0 50.0 55.1 57.6 408-440 bp 1.0 62.3
ccsA-ndhD_p5 TTGGGGTCAATCTCTTAGGA ACTAGCAAGTGACAAGGTCG 45.0 50.0 55.1 57.6 409-441 bp 1.0 62.3