Hotspot

ndhF-rpl32

SSC · 113125-114044 · 920 bp

Back to Maerua

Pi 0.0272
MarkerSeek score 66.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
920
variable_sites
24
pi
0.0272
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TCCTTGTTGCTGTCGGAAAA ACGCTTTTTCGAAATAGAAGTACG 45.0 37.5 58.0 58.2 1065-1077 bp 1.0 51.1
ndhF-rpl32_p2 TCCTTGTTGCTGTCGGAAAA CGCTTTTTCGAAATAGAAGTACGT 45.0 37.5 58.0 58.2 1064-1076 bp 1.0 51.1
ndhF-rpl32_p3 TTCCTTGTTGCTGTCGGAAA ACGCTTTTTCGAAATAGAAGTACG 45.0 37.5 58.0 58.2 1066-1078 bp 1.0 51.1
ndhF-rpl32_p4 TTCCTTGTTGCTGTCGGAAA CGCTTTTTCGAAATAGAAGTACGT 45.0 37.5 58.0 58.2 1065-1077 bp 1.0 51.1
ndhF-rpl32_p5 AGATTCCTTGTTGCTGTCGG CGCTTTTTCGAAATAGAAGTACGT 50.0 37.5 58.2 58.2 1068-1080 bp 1.0 49.9