Hotspot

rps4-trnT-UGU

LSC · 46645-47029 · 385 bp

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Pi 0.0247
MarkerSeek score 69.30
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
385
variable_sites
9
pi
0.0247
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 CGGAGCGTGATTGGTTTCTA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 544-563 bp 1.0 83.2
rps4-trnT-UGU_p2 CGGAGCGTGATTGGTTTCTA ATGGTCATCGGTTCGATTCC 50.0 50.0 58.0 57.8 512-531 bp 1.0 82.6
rps4-trnT-UGU_p3 CGGAGCGTGATTGGTTTCTA ATTTCGGTTAGTTGAGCCCG 50.0 50.0 58.0 58.3 569-588 bp 1.0 82.0
rps4-trnT-UGU_p4 CGGAGCGTGATTGGTTTCTA GGTTAGTTGAGCCCGCTTAG 50.0 55.0 58.0 58.3 564-583 bp 1.0 81.4
rps4-trnT-UGU_p5 CGGAGCGTGATTGGTTTCTA CCGCTTAGCTCAGAGGTTAG 50.0 55.0 58.0 57.5 552-571 bp 1.0 80.2