Hotspot

trnG-UCC-trnfM-CAU

LSC · 37359-37497 · 139 bp

Back to Maerua

Pi 0.0511
MarkerSeek score 74.00
Primer available yes
Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name
trnG-UCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
139
variable_sites
7
pi
0.0511
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnfM-CAU_p1 TCTTTGCCAAGGAGAAGACG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 217-219 bp 1.0 81.6
trnG-UCC-trnfM-CAU_p2 TCTTTGCCAAGGAGAAGACG TTCTTTTCATCGCGGGGTAG 50.0 50.0 57.8 58.0 267-269 bp 1.0 81.5
trnG-UCC-trnfM-CAU_p3 TCTTTGCCAAGGAGAAGACG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 253-255 bp 1.0 81.3
trnG-UCC-trnfM-CAU_p4 TCTTTGCCAAGGAGAAGACG GTCACGGGTTCAAATCCTGT 50.0 50.0 57.8 58.1 211-213 bp 1.0 81.2
trnG-UCC-trnfM-CAU_p5 TCTTTGCCAAGGAGAAGACG TCACGGGTTCAAATCCTGTC 50.0 50.0 57.8 57.8 210-212 bp 1.0 80.6