Hotspot

trnS-UGA-psbZ

LSC · 36380-36723 · 344 bp

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Pi 0.0267
MarkerSeek score 66.90
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
344
variable_sites
9
pi
0.0267
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 TTTTCAAGACCGGAGCCATC CCAACCATCAGGAGAAGCAA 50.0 50.0 58.2 57.8 477-483 bp 1.0 79.6
trnS-UGA-psbZ_p2 TTTTCAAGACCGGAGCCATC AACCAACCATCAGGAGAAGC 50.0 50.0 58.2 57.8 479-485 bp 1.0 79.6
trnS-UGA-psbZ_p3 GTTTTCAAGACCGGAGCCAT CCAACCATCAGGAGAAGCAA 50.0 50.0 58.5 57.8 478-484 bp 1.0 77.3
trnS-UGA-psbZ_p4 GTTTTCAAGACCGGAGCCAT AACCAACCATCAGGAGAAGC 50.0 50.0 58.5 57.8 480-486 bp 1.0 77.3
trnS-UGA-psbZ_p5 TTTTCAAGACCGGAGCCATC ACTCAACCAACCATCAGGAG 50.0 50.0 58.2 57.1 483-489 bp 1.0 74.3