Hotspot

trnM-CAU-psbD

LSC · 32289-33673 · 1385 bp

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Pi 0.0256
MarkerSeek score 67.70
Primer available yes
Label trnM-CAU-psbD

Feature Metadata

label_name
trnM-CAU-psbD
feature_type
igs
region
LSC
length_bp
1385
variable_sites
34
pi
0.0256
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-psbD_p1 GGCGTAAGTCATCGGTTCAA TTTACCAAGGGCTATAGTCA 50.0 40.0 58.3 53.0 1384-1434 bp 1.0 45.2
trnM-CAU-psbD_p2 CGCTATGGTAAGGCGTAAGT TTTACCAAGGGCTATAGTCA 50.0 40.0 57.5 53.0 1395-1445 bp 1.0 45.2
trnM-CAU-psbD_p3 GGCGTAAGTCATCGGTTCAA TTACCAAGGGCTATAGTCAT 50.0 40.0 58.3 52.8 1383-1433 bp 1.0 45.2
trnM-CAU-psbD_p4 GGCCCTTTTAACTCAGTGGT TTTACCAAGGGCTATAGTCA 50.0 40.0 57.7 53.0 1422-1472 bp 1.0 45.1
trnM-CAU-psbD_p5 GGCCCTTTTAACTCAGTGGT TTACCAAGGGCTATAGTCAT 50.0 40.0 57.7 52.8 1421-1471 bp 1.0 45.1