Hotspot

trnE-UUC-trnT-GGU

LSC · 31468-32223 · 756 bp

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Pi 0.0294
MarkerSeek score 68.90
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
756
variable_sites
22
pi
0.0294
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 897-902 bp 1.0 81.3
trnE-UUC-trnT-GGU_p2 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 859-864 bp 1.0 80.5
trnE-UUC-trnT-GGU_p3 TGTCCTGAACCACTAGACGA GAACCGATGACTTACGCCTT 50.0 50.0 57.7 58.0 838-843 bp 1.0 80.5
trnE-UUC-trnT-GGU_p4 TTTCGTAGTACCCTACCCCC CCGATGACTTACGCCTTACC 55.0 55.0 57.8 58.2 894-899 bp 1.0 80.1
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG CCGATGACTTACGCCTTACC 55.0 55.0 58.3 58.2 856-861 bp 1.0 79.4