Hotspot

petN-psbM

LSC · 28971-29604 · 634 bp

Back to Maerua

Pi 0.0221
MarkerSeek score 66.00
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
634
variable_sites
14
pi
0.0221
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCATGGGCT GCACTCTTCATTCTCGTTCCT 50.0 47.6 58.2 58.0 789-795 bp 1.0 71.4
petN-psbM_p2 TAGTAAGTCTCGCATGGGCT ACTGCACTCTTCATTCTCGT 50.0 45.0 58.2 56.9 792-798 bp 1.0 70.7
petN-psbM_p3 ATGGGGAAGAAGTGGACTCT GCACTCTTCATTCTCGTTCCT 50.0 47.6 57.6 58.0 728-734 bp 1.0 70.5
petN-psbM_p4 CCCTCGTAGTATGGGGAAGA GCACTCTTCATTCTCGTTCCT 55.0 47.6 57.6 58.0 738-744 bp 1.0 70.2
petN-psbM_p5 TCCCTCGTAGTATGGGGAAG GCACTCTTCATTCTCGTTCCT 55.0 47.6 57.6 58.0 739-745 bp 1.0 70.2