Hotspot

trnH-GUG-psbA

LSC · 127-535 · 409 bp

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Pi 0.0645
MarkerSeek score 74.20
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
409
variable_sites
24
pi
0.0645
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GGTGTAGCTGGTGTATTCGG 55.0 55.0 58.1 58.1 866-893 bp 1.0 84.4
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GGTGTAGCTGGTGTATTCGG 50.0 55.0 57.9 58.1 880-907 bp 1.0 84.2
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC GGTGTAGCTGGTGTATTCGG 50.0 55.0 57.9 58.1 879-906 bp 1.0 84.2
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GGTGTAGCTGGTGTATTCGG 50.0 55.0 57.9 58.1 881-908 bp 1.0 84.2
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CCTCTAGACCTAGCAGCTGT 55.0 55.0 58.1 57.9 476 bp 0.5 58.4