Hotspot

rpl16

LSC · 78203-79630 · 1428 bp

Back to Macrotyloma

Pi 0.00607
MarkerSeek score 36.60
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1428
variable_sites
13
pi
0.00607
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 ATCGCCGATTACTAGCTCCT CTCAGGTGTCGAGGGAATTG 50.0 55.0 58.1 58.0 2293-2294 bp 1.0 79.9
rpl16_p2 ATCGCCGATTACTAGCTCCT ATTGCGCGTGTAGAATGGAT 50.0 45.0 58.1 58.0 1938-1939 bp 1.0 79.8
rpl16_p3 ACCTCGGGTGCTAATGAAAC CTCAGGTGTCGAGGGAATTG 50.0 55.0 57.9 58.0 2561-2562 bp 1.0 79.6
rpl16_p4 CGGATGTAGCCAAGTGGATC CTCAGGTGTCGAGGGAATTG 55.0 55.0 58.1 58.0 2795-2796 bp 1.0 79.5
rpl16_p5 TGGATCAAGGCAGTGGATTG CTCAGGTGTCGAGGGAATTG 50.0 55.0 57.9 58.0 2781-2782 bp 1.0 79.4