Hotspot

psbC

LSC · 50432-51853 · 1422 bp

Back to Macrotyloma

Pi 0.000938

MarkerSeek score 41.40

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 2
pi: 0.000938
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	GAAGGTATTCGTGCGTGGAT	ACGACTCGCTCAACTCAATC	50.0	50.0	58.1	58.0	1531 bp	1.0	79.5
psbC_p2	GTTTCTCAAGAAATCCGCGC	ACGACTCGCTCAACTCAATC	50.0	50.0	58.1	58.0	1603 bp	1.0	79.0
psbC_p3	CGTTTCTCAAGAAATCCGCG	ACGACTCGCTCAACTCAATC	50.0	50.0	57.9	58.0	1604 bp	1.0	78.8
psbC_p4	GCAGCGGAAGATCCTGAATT	ACGACTCGCTCAACTCAATC	50.0	50.0	58.3	58.0	1585 bp	1.0	77.4
psbC_p5	AAGGTATTCGTGCGTGGATG	ACGACTCGCTCAACTCAATC	50.0	50.0	58.3	58.0	1530 bp	1.0	77.2