Hotspot

ndhA

SSC · 113651-115999 · 2349 bp

Back to Macrotyloma

Pi 0.00539

MarkerSeek score 46.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2349
variable_sites: 19
pi: 0.00539
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AATTCATCCGCCGGGTTTTA	GTACCTAGCAGCTCGGACTA	45.0	55.0	57.9	58.0	2627 bp	1.0	79.3
ndhA_p2	AATTCATCCGCCGGGTTTTA	ACCTAGCAGCTCGGACTATT	45.0	50.0	57.9	57.9	2625 bp	1.0	79.0
ndhA_p3	AATTCATCCGCCGGGTTTTA	CAATGTACCTAGCAGCTCGG	45.0	55.0	57.9	58.1	2631 bp	1.0	78.5
ndhA_p4	AATTCATCCGCCGGGTTTTA	CCAATGTACCTAGCAGCTCG	45.0	55.0	57.9	58.1	2632 bp	1.0	78.5
ndhA_p5	AACAAGCTTTGGAAGGCCTT	GTACCTAGCAGCTCGGACTA	45.0	55.0	58.2	58.0	2859 bp	0.666667	64.9