Hotspot

ccsA

SSC · 120369-121355 · 987 bp

Back to Macrotyloma

Pi 0.00608

MarkerSeek score 47.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 987
variable_sites: 9
pi: 0.00608
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTCGACGAAATGGGTGGAAT	CTCTTAGGAAGCAGTGCTGG	50.0	55.0	58.0	58.0	1816-1818 bp	1.0	80.4
ccsA_p2	CTCGACGAAATGGGTGGAAT	CTTAGGAAGCAGTGCTGGAG	50.0	55.0	58.0	58.0	1814-1816 bp	1.0	80.4
ccsA_p3	TTTCGGCTGACAAGGTTGAA	CTCTTAGGAAGCAGTGCTGG	45.0	55.0	58.0	58.0	1443-1445 bp	1.0	80.4
ccsA_p4	TTTCGGCTGACAAGGTTGAA	CTTAGGAAGCAGTGCTGGAG	45.0	55.0	58.0	58.0	1441-1443 bp	1.0	80.4
ccsA_p5	TTTTCGGCTGACAAGGTTGA	CTTAGGAAGCAGTGCTGGAG	45.0	55.0	58.0	58.0	1442-1444 bp	1.0	80.4