Hotspot

accD

LSC · 20804-22309 · 1506 bp

Back to Macrotyloma

Pi 0.00354

MarkerSeek score 60.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1506
variable_sites: 8
pi: 0.00354
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CCTGTATGTTTTCATTTTTGATTGCA	TCATTTCTTTTGAATACTTCCTCA	30.8	29.2	57.9	54.5	1644 bp	1.0	40.4
accD_p2	CCTGTATGTTTTCATTTTTGATTGCA	ACTTCCTCATTCTTTATTCTTTTCAGT	30.8	29.6	57.9	57.4	1629 bp	1.0	40.4
accD_p3	CCTGTATGTTTTCATTTTTGATTGCA	TCTTTTGAATACTTCCTCATTCT	30.8	30.4	57.9	53.4	1639 bp	1.0	40.4
accD_p4	TCCTGTATGTTTTCATTTTTGATTGC	TCATTTCTTTTGAATACTTCCTCA	30.8	29.2	57.7	54.5	1645 bp	1.0	40.4
accD_p5	CCTGTATGTTTTCATTTTTGATTGCA	TCTTTTCAGTTACTAATCCTCA	30.8	31.8	57.9	52.2	1612 bp	1.0	40.4